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. 2012 Apr 19;7(4):e35887. doi: 10.1371/journal.pone.0035887

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The newly designed primers were used in PCR reactions for individuals belonging to various groups within six phyla: Sipuncula- 1 Phascolosoma sp.; Echinodermata- 2 Ophiocoma erinaceus (brittlestar); Chordata- 3 Pseudamiops gracilicauda (fish); Annelida (Polychaeta)- 4 Pherecardia striata, 5 unidentified terebellid species; Arthropoda- 6 Xanthias latifrons (brachyuran crab), 7 Pilodius flavus (brachyuran crab), 8 Liomera sp. (brachyuran crab), 9 Carupa sp. (brachyuran crab), 10 unidentified pilumnid species (brachyuran crab), 11 unidentified xanthid species (brachyuran crab), 12 Calcinus gouti (anomuran crab), 13 Synalpheus sp. (caridean shrimp), 14 Periclimenes sp. (caridean shrimp), 15 unidentified caridean shrimp, 16 unidentified amphipod species; Mollusca- 17 Cypraea helvola (gastropod), 18 Cypraea fimbriata (gastropod), 19 Trivia sp. (gastropod), 20 Erato sandwichensis (gastropod), 21 unidentified haminoeid species (gastropod), 22 Berthellina sp. (gastropod), 23 Chlamys sp. (bivalve), 24 Lima sp. (bivalve), 25 unidentified lucinid species (bivalve). nc-negative control. Good amplifications were observed for all individuals except one bivalve (24).