Table 2. Selected colonies and detailed quantitative and qualitative overview of the SDH substitutions selected with different carboxamides concentrations.
| Compound | Carboxin | Isopyrazam | compound A | Fluopyram | Boscalid | ||||||
| Concentration for selection | MSC | 5× MSC | MSC | 5× MSC | MSC | 2.5× MSC | MSC | 5× MSC | MSC | 5× MSC | |
| False positives | 12 | - | 9 | - | 6 | - | 3 | - | - | - | |
| True positives | 109 | 2 | 63 | 22 | 33 | 29 | 128 | 2 | 50 | 14 | |
| No SDH substitution | 3 | - | - | - | 5 | - | - | - | - | - | |
| With SDH substitution | 106 | 2 | 63 | 22 | 28 | 29 | 128 | 2 | 50 | 14 | |
| Codons | Substitution | ||||||||||
| tct>ttt | SDHB_S218F | - | - | - | - | - | - | 1 | - | - | - |
| cca>aca | SDHB_P220T | - | - | - | - | - | - | 1 | - | - | - |
| cca>cta | SDHB_P220L | - | - | - | - | - | - | 2 | - | - | - |
| tcc>ccc | SDHB_S221P | 2 | - | - | - | - | - | - | - | - | - |
| aac>cac | SDHB_N225H | 1 | - | - | - | - | - | - | - | - | - |
| aac>atc | SDHB_N225I | - | - | - | - | - | - | 1 | - | - | - |
| cga>cca | SDHB_R265P | 4 | - | - | - | - | - | - | - | - | - |
| cac>cta/ctc/ctt | SDHB_H267L | 7 | 2 | 16 | 3 | 2 | - | 7 | - | 6 | - |
| cac>aac | SDHB_H267N | 11 | - | - | - | - | - | - | - | - | - |
| cac>cag | SDHB_H267Q | - | - | - | - | - | - | - | - | - | 2 |
| cac>tac | SDHB_H267Y | 61 | - | - | - | - | - | - | - | 38 | 5 |
| att>gtt | SDHB_I269V | 11 | - | - | - | - | - | - | - | - | - |
| aac>aag | SDHB_N271K | - | - | - | - | - | - | 6 | - | - | - |
| acc>atc | SDHC_T79I | - | - | - | - | - | - | - | - | - | 4 |
| tcg>ggg | SDHC_S83G | - | - | 1 | - | - | - | 4 | 2 | - | - |
| gcc>gtc/gtt | SDHC_A84V | - | - | - | - | - | - | 73 | - | - | - |
| gcc>atc | SDHC_A84I | - | - | - | - | - | - | 1 | - | - | - |
| ctc>ccc | SDHC_L85P | 2 | - | - | - | - | - | - | - | - | - |
| acc>aag/aaa | SDHC_N86K | - | - | 8 | 3 | 4 | 9 | 6 | - | - | 1 |
| cgc>tgc | SDHC_R87C | - | - | - | - | - | - | 24 | - | - | - |
| gac>gtc | SDHC_V88D | - | - | - | - | 1 | - | - | - | - | - |
| cac>cgt | SDHC_H145R | 1 | - | - | - | - | - | - | - | - | - |
| cat>cgt/cga | SDHC_H152R | - | - | 37 | 16 | 21 | 20 | - | - | - | 1 |
| gac>gag/gaa | SDHD_D129E | 2 | - | - | - | - | - | - | - | 2 | - |
| gac>ggc | SDHD_D129G | 2 | - | 1 | - | - | - | - | - | 1 | 1 |
| gac>tcc | SDHD_D129S | 1 | - | - | - | - | - | - | - | - | - |
| gac>acc | SDHD_D129T | - | - | - | - | - | - | 1 | - | - | - |
| Multiple SDHB | - | - | - | - | - | - | 1 | - | - | - | |
| Multiple SDHC | - | - | - | - | - | - | - | - | 3 | - | |
| Multiple SDHD | 1 | - | - | - | - | - | - | - | - | - | |
Numbers correspond to the total number of colonies belonging to each sub-group (true/false positives and with/without mutation in the SDH encoding genes) for each selection condition (See material and methods). The second part details the number of colonies for the 27 SDH substitution type selected on each carboxamide concentration.