Table 4. Enzymatic efficiencies determinations for selected subset of strains representative of each mutation type.
| Mutation | Strain | Covalent FADa | MTT/PMS activityb | Q0/DCPIP activityc | ||||
| none | IPO323 | 330.3±23.6 | 474 | ±62 | (100%) | 484.4154407 | ±42 | (100%) |
| B_S218F | Flu41 | 344.4±14 | 416 | ±67 | (88%) | 92.87292759 | ±18 | (19%) |
| B_P220T | Flu110 | 378.0±4.7 | 720 | ±110 | (152%) | 107.7682646 | ±9 | (22%) |
| B_P220L | Flu75 | 304.5±5.8 | 717 | ±84 | (151%) | 231.8025324 | ±16 | (48%) |
| B_N225H | Cbx106 | 299.5±3.8 | 850 | ±175 | (179%) | 334.8801055 | ±33 | (69%) |
| B_N225I | Flu7 | 281.0±14.2 | 651 | ±98 | (137%) | 188.7997691 | ±19 | (39%) |
| B_R265P | Cbx56 | 363.1±19.7 | 217 | ±53 | (46%) | 41.7782216 | ±10 | (9%) |
| B_H267L | Cbx29 | 519.5±10.4 | 381 | ±37 | (80%) | 64.57824113 | ±7 | (13%) |
| B_H267N | Cbx96 | 246.7±4.3 | 553 | ±77 | (117%) | 94.85037408 | ±15 | (20%) |
| B_H267Q | Bos59 | 453.8±8.5 | 334 | ±40 | (70%) | 65.25108944 | ±6 | (13%) |
| B_H267Y | Cbx28 | 438.4±25.5 | 190 | ±44 | (40%) | 45.65897328 | ±18 | (9%) |
| B_I269V | Cbx1 | 387.2±21.0 | 545 | ±147 | (115%) | 416.7898614 | ±73 | (86%) |
| B_N271K | Flu33 | 286.9±7.8 | 218 | ±26 | (46%) | 25.98331188 | ±5 | (5%) |
| C_T79I | Bos64 | 241.9±3.0 | 501 | ±70 | (106%) | 124.887427 | ±16 | (26%) |
| C_S83G | Flu1 | 368.6±12.4 | 714 | ±97 | (151%) | 286.9379225 | ±34 | (59%) |
| C_A84V | Flu4 | 229.6±9.1 | 850 | ±119 | (179%) | 419.0858506 | ±59 | (87%) |
| C_A84I | Flu21 | 631.5±15.4 | 596 | ±95 | (126%) | 245.6437942 | ±32 | (51%) |
| C_L85P | Cbx12 | 320.8±24.9 | 334 | ±56 | (70%) | 143.5533934 | ±28 | (30%) |
| C_N86K | Izm7 | 284.7±6.6 | 407 | ±86 | (86%) | 200.6388817 | ±35 | (41%) |
| C_R87C | Flu9 | 333.5±9.3 | 389 | ±46 | (82%) | 117.638248 | ±12 | (24%) |
| C_V88D | Ol50 | 200.5±7.8 | 838 | ±170 | (177%) | 139.7192089 | ±21 | (29%) |
| C_H145R | Cbx76 | 377.8±27.2 | 555 | ±98 | (117%) | 97.84219544 | ±12 | (20%) |
| C_H152R | Izm1 | 156.7±8.8 | 227 | ±34 | (48%) | 106.7682223 | ±22 | (22%) |
| D_D129E | Cbx22 | 211.4±7.6 | 491 | ±123 | (104%) | 202.5551707 | ±30 | (42%) |
| D_D129G | Cbx67 | 180.9±3.8 | 808 | ±80 | (171%) | 91.9077442 | ±9 | (19%) |
| D_D129S | Cbx101 | 453.8±8.5 | 534 | ±84 | (113%) | 111.2427749 | ±17 | (23%) |
| D_D129T | Flu29 | 253.9±10.5 | 568 | ±69 | (120%) | 29.33687605 | ±6 | (6%) |
a
Covalent FAD is expressed in pmol covalend FAD mg of protein−1 and is an estimation of the total amount of succinate dehydrogenase enzyme present in the mitochondrial preparation, presented values are the average of four individual determinations ± S.D.
b
MTT/PMS activity expressed as µmol PMS reduced MTT min−1 µmol covalent FAD−1. Values represent the means of at least 10 determinations ± S.D.
c
Qo mediated DCPIP reduction expressed in µmol reduced DCPIP min−1 µmol covalent FAD−1 in the presence of 1 mM Qo. Values represent the means of at least 10 determinations ± S.D.