Figure 1. Generation and genotyping of Btbd9 mutant mice.

(A) The normal wild-type (WT) allele of the Btbd9 gene contains 12 exons. A commercially available mutant allele of the Btbd9 gene has a β-geo gene trap inserted into the sixth intron. Filled vertical rectangles represent coding exons; open vertical rectangles represent non-coding exons. (B) To genotype the mice a two-step process was taken. First, primers were used to detect specifically the β-geo gene trap vector, which produced a fragment of approximately 311 bp in length with an internal control fragment of approximately 200 bp in length. Second, primers were used to detect specifically a WT allele, which would produce a fragment of approximately 500 bp in length and an internal control β-geo fragment of 311 bp in length.