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. 2012 Apr 19;7(4):e35285. doi: 10.1371/journal.pone.0035285

Figure 5. Fluorescence titrations of SsbB.

Figure 5

A) 0.4 µM SsbB was titrated with increasing concentrations of poly(dT) in a buffer containing 20 mM Tris pH 7.5 and either 20 mM NaCl (open circles), 200 mM NaCl (close circles) and 500 mM NaCl (closed triangles). B) 0.4 µM SsbB was titrated with increasing concentrations of dTn of 25 (closed circles), 35 (open circles) and 45 (closed triangles) nucleotides in a buffer containing 20 mM Tris pH 7.5 and 200 mM NaCl.