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. 2012 Apr 20;7(4):e35630. doi: 10.1371/journal.pone.0035630

Figure 1. SPR sensograms and fits for PKCε activating VHHs.

Figure 1

SPR sensograms and fits for second-order Langmuir binding models are shown for VHHs A10 (A), C1 (B) and D1 (C). The VHH injection time was 3 min, followed by a dissociation time of 5 min. The surface was regenerated with an injection of 10 mM NaOH for 3 min, followed by a stabilization time of 5 min between each VHH injection. Five concentrations of each VHH were used, with the middle concentration injected twice as an internal control. The VHH concentrations (in µg/ml) are marked adjacent to each fit on the right hand side of the figure.