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The activity of the catalytic domain of PKCε was measured with varying MARCKS substrate concentrations and varying concentrations of VHHs E6 (A) and G8 (B). The data was analyzed using non-linear regression and the Michaelis-Menten kinetics model and represents 3 independent experiments, each with duplicates. The data is presented as a Lineweaver-Burk plot to allow for the easy visualization of Km and Vmax values.
