Table 1. Association and dissociation constants for the interaction of VHHs with PKCε obtained from SPR measurements.
VHH | ka 1 (1/(M*s)) | kd 1 (1/s) | KD 1 (M) | ka 2 (1/(M*s)) | kd 2 (1/s) | KD 2 (M) |
A10 | 2.95×101 (±4.31×102) | 7.50×10−4 (±7.62×10−5) | 2.54×10−5 (±1.76×10−6) | 1.01×103 (±1.98×103) | 1.05×10−1 (±3.27×10−6) | 1.04E×10−4 (±7.21×10−5) |
C1 | 3.09×102 (±1.60×102) | 1.04×10−3 (±3.56×10−5) | 3.38×10−6 (±2.56×10−6) | 1.12×104 (±4.11×103) | 8.21×10−2 (±4.10×10−6) | 7.30×10−6 (±3.08×10−6) |
D1 | 2.41×101 (±2.89×10−1) | 1.07×10−3 (±2.00×10−4) | 4.42×10−5 (±8.80×10−6) | 5.51×103 (±1.27) | 4.35×10−2 (±9.02×10−5) | 7.91×10−6 (±1.82×10−8) |
E6 | 5.22×102 (±2.30×102) | 3.06×10−4 (±6.60×10−5) | 5.87×10−7 (±4.78×10−7) | 1.53×103 (±4.59×103) | 1.49×10−2 (±5.10×10−6) | 9.71×10−6 (±3.64×10−6) |
G8 | 1.11×101 (±7.11×102) | 1.13×10−3 (±4.01×10−5) | 1.02×10−4 (±1.58×10−6) | n/a | n/a | n/a |