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. 2012 Apr 20;7(4):e35630. doi: 10.1371/journal.pone.0035630

Table 1. Association and dissociation constants for the interaction of VHHs with PKCε obtained from SPR measurements.

VHH ka 1 (1/(M*s)) kd 1 (1/s) KD 1 (M) ka 2 (1/(M*s)) kd 2 (1/s) KD 2 (M)
A10 2.95×101 (±4.31×102) 7.50×10−4 (±7.62×10−5) 2.54×10−5 (±1.76×10−6) 1.01×103 (±1.98×103) 1.05×10−1 (±3.27×10−6) 1.04E×10−4 (±7.21×10−5)
C1 3.09×102 (±1.60×102) 1.04×10−3 (±3.56×10−5) 3.38×10−6 (±2.56×10−6) 1.12×104 (±4.11×103) 8.21×10−2 (±4.10×10−6) 7.30×10−6 (±3.08×10−6)
D1 2.41×101 (±2.89×10−1) 1.07×10−3 (±2.00×10−4) 4.42×10−5 (±8.80×10−6) 5.51×103 (±1.27) 4.35×10−2 (±9.02×10−5) 7.91×10−6 (±1.82×10−8)
E6 5.22×102 (±2.30×102) 3.06×10−4 (±6.60×10−5) 5.87×10−7 (±4.78×10−7) 1.53×103 (±4.59×103) 1.49×10−2 (±5.10×10−6) 9.71×10−6 (±3.64×10−6)
G8 1.11×101 (±7.11×102) 1.13×10−3 (±4.01×10−5) 1.02×10−4 (±1.58×10−6) n/a n/a n/a