Table 2. Km and Vmax values for PKCε activating and inhibiting VHHs.
PKCε with activators PS and DOG (n≥3) | PKCε without activators PS and DOG (n≥3) | Catalytic domain (n≥2) | ||||
Km (µM) | Vmax | Km (µM) | Vmax | Km (µM) | Vmax | |
Control | 424 | 141 | 449 | 139 | 130 | 120 |
A10 | 348 | 253 | 352 | 263 | no effect | no effect |
C1 | 81 | 191 | 105 | 139 | no effect | no effect |
D1 | 126 | 158 | 137 | 208 | no effect | no effect |
E6 | 17 | 29 | 5.1 | 24 | n/a | 6.7 |
G8 | 260 | 113 | 121 | 71 | 158 | 43 |