Table 1. All identified polymorphisms with their location within motilin (MLN).
SNP/microsatellite | I | Location of polymorphism in MLN | Primer F (5′>3′) | Primer R (5′>3′) | AT | PS |
FN298674:g.62G>A | + | promoter | CAGGAATCCAGACTCCTCAC | CCGGCTCTTTTGTTAACTT | 60 | 521 |
FN298674:g.90T>C | + | exon 1 | CAGGAATCCAGACTCCTCAC | CCGGCTCTTTTGTTAACTT | 60 | 521 |
FN298674:g.551(AG)45 | + | intron 1 | GCTCCGGGAAGTTAACAAAA | GAGCTGCTGACTTTGCCTTT * | 59 | 241 |
FN298674:g.658A>G | intron 1 | GCTCCGGGAAGTTAACAAAA | ACATTTAATTCACCCATAAGAATCAA | 59 | 403 | |
FN298674:g.684G>A | intron 1 | GCTCCGGGAAGTTAACAAAA | ACATTTAATTCACCCATAAGAATCAA | 59 | 403 | |
FN298674:g.707G>A | intron 1 | GCTCCGGGAAGTTAACAAAA | ACATTTAATTCACCCATAAGAATCAA | 59 | 403 | |
FN298674:g.869T>A | intron 1 | GAAGTTAACAAAAGAGCCGG | ACCCTCCCTTTGGAGAGAAT | 60 | 822 | |
FN298674:g.1052C>T | intron 1 | GAAGTTAACAAAAGAGCCGG | ACCCTCCCTTTGGAGAGAAT | 60 | 822 | |
FN298674:g.1719A>T | intron 1 | GCAGCCTTGGAAGATTCTCT | CATCCCATAAGTGGGTGTGT | 60 | 591 | |
FN298674:g.1891insG | + | intron 1 | GACACACCCACTTATGGGAT | TCCCAGCTCCAGCAAGTCTA * | 60 | 155 |
FN298674:g.2037T>C | intron 1 | GACACACCCACTTATGGGAT | TTGTTGGCCCTTCCCTTTG | 58 | 514 | |
FN298674:g.2045C>G | + | intron 1 | GACACACCCACTTATGGGAT | TTGTTGGCCCTTCCCTTTG | 58 | 514 |
FN298674:g.2451T>A | intron 1 | GCTCCTGCTTCATTAGCTC | GTGTGTCTGACCTCTCACC | 58 | 779 | |
FN298674:g.2525G>T | intron 1 | GCTCCTGCTTCATTAGCTC | GTGTGTCTGACCTCTCACC | 58 | 779 | |
FN298674:g.2929G>A | + | intron 2 | GCTCCTGCTTCATTAGCTC | GTGTGTCTGACCTCTCACC | 58 | 779 |
FN298674:g.2985C>T | + | intron 2 | GCTCCTGCTTCATTAGCTC | GTGTGTCTGACCTCTCACC | 58 | 779 |
FN298674:g.3537(T)23 | intron 2 | CCCTGGGCCTTTTACCTTAG * | TTGCCATTTCCTTTTCCAAC | 60 | 130 | |
FN298674:g.4189C>T | intron 2 | GAGACTGAGGCTCCAACAGG | CTGGCAATTACGAGGAGGAA | 60 | 503 | |
FN298674:g.4360G>C | + | intron 2 | GAGACTGAGGCTCCAACAGG | CTGGCAATTACGAGGAGGAA | 60 | 503 |
FN298674:g.4942insT | + | intron 2 | AATCCGAGGACGATGTGAAG * | GCCCTTTGGGGTTTCTATTC | 60 | 128 |
FN298674:g.5224G>T | intron 3 | GCAGCGAGAGGGTGCTTG | AGCTCGTCACCTGAGTGTC | 58 | 795 | |
FN298674:g.5596T>G | intron 3 | CACAGAATGTGCTGCGAACT | AATGGATCTCATGGCTCTGG | 60 | 460 | |
FN298674:g.6197G>A | intron 3 | GCCCTTCTCCCTCAGCTACT | CCAATTTCCACAGGAGCAGT | 60 | 479 | |
FN298674:g.6689C>T | + | intron 4 | ACGCTGCCTTATTTCACAGC | ATGGATCTCTGCAGGAGGAG | 60 | 496 |
FN298674:g.6728G>A | + | intron 4 | CTGGCAGGTTTCAGGCTCT | ATGGATCTCTGCAGGAGGAG | 58 | 672 |
FN298674:g.6920C>T | intron 4 | CTGGCAGGTTTCAGGCTCT | ATGGATCTCTGCAGGAGGAG | 58 | 672 | |
FN298674:g.6984C>T | intron 4 | CTGGCAGGTTTCAGGCTCT | ATGGATCTCTGCAGGAGGAG | 58 | 672 | |
FN298674:g.7002T>A | intron 4 | TCCGAGCGGAGTCTAATCAC | CTGCCTCCAGCTTTCTGTGT | 60 | 533 | |
FN298674:g.7153C>T | intron 4 | TCCGAGCGGAGTCTAATCAC | CTGCCTCCAGCTTTCTGTGT | 60 | 533 | |
FN298674:g.7403delGAC | intron 4 | CAGCCTGAGTCACTGGTAC | AGCTCAGAAGGGTCAGCAGATC * | 60 | 245 | |
FN298674:g.7408delA | intron 4 | CAGCCTGAGTCACTGGTAC | AGCTCAGAAGGGTCAGCAGATC * | 60 | 245 | |
FN298674:g.7410TCA>CTG | intron 4 | CAGCCTGAGTCACTGGTAC | ATTTATGGCCAAACCCTCTC | 58 | 528 |
IRD labeled.
Informative (I) polymorphisms are indicated by a plus sign. The primer pairs used are given with their annealing temperatures (AT) and product sizes (PS).