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. 2012 Apr 20;7(4):e36020. doi: 10.1371/journal.pone.0036020

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Mahla et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Total RNA was extracted from prepubertal (4- to 6- month old; lanes 1, 3, 5) and from adult (2- to 3- year old; lanes 2, 4, 6) buffalo testes and pooled for template. Transcript of POU5F1 was detected both in prepubertal (lane 1) and adult (lane 2) testes. BETA-ACTIN was used for normalizing RNA samples in prepubertal (lanes 3) and adult (lane 4) testes. Isolated RNA without reverse transcription to check genomic DNA contamination in prepubertal (lane5) and adult (lane 6) testes. M: 100 base pair molecular weight marker.