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. 2012 Apr 20;7(4):e35683. doi: 10.1371/journal.pone.0035683

Figure 3. Changes in GIPR mRNA levels in macrophages and pancreatic β-cells ex vivo and in vitro.

Figure 3

A, Exudate peritoneal macrophages were obtained from 5 nondiabetic mice and 5 STZ-induced diabetic Apoe −/− mice by intraperitoneal injection of thioglycolate. B, Exudate peritoneal macrophages were obtained from 3 db/misty mice and 3 db/db mice at 12 weeks of age. C, THP-1 macrophages (human), J774A1 macrophages (mouse), and 1.2B4 pancreatic β-cells (human) were incubated with RPMI-1640 containing high glucose (500 mg/dl) for 9–10 days. Changes in GIPR mRNA levels were determined by real-time RT-PCR. Three independent experiments were performed. *P<0.05, **P<0.01 vs. each baseline of day 0.