Table 1. Effect of preincubation time on TR activity inhibition of TGR.
Preincubation time (min) | Inhibition % (*) |
3 | 48 |
30 | 82 |
60 | 92 |
90 | 96 |
120 | 100 |
1.5 µM of compound 1 was incubated with 1 nM enzyme in the presence of NADPH and the reaction started by the addition of DTNB. Control experiments without inhibitors were carried out in parallel to determine the corresponding vo (initial velocities without inhibitors).