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. 2012 Apr 20;7(4):e35335. doi: 10.1371/journal.pone.0035335

Table 2. PCR amplification of transgene DNA sequences in piglets.

Lentiviruses (5×105 ifu/ml, ml) Number of sperm incubated with lentiviruses Source of DNA template for PCR amplification
Ear biopsy from 3 day old piglet Ear biopsy from 30 day old piglet Ear biopsy from 120 day old piglet
No. of piglets No. of PCR-positive piglets (%) No. of PCR-positive piglets No. of PCR-positive piglets
10 1×109 59 6 (10.17%) 6 6