Table 2. PCR amplification of transgene DNA sequences in piglets.
Lentiviruses (5×105 ifu/ml, ml) | Number of sperm incubated with lentiviruses | Source of DNA template for PCR amplification | |||
Ear biopsy from 3 day old piglet | Ear biopsy from 30 day old piglet | Ear biopsy from 120 day old piglet | |||
No. of piglets | No. of PCR-positive piglets (%) | No. of PCR-positive piglets | No. of PCR-positive piglets | ||
10 | 1×109 | 59 | 6 (10.17%) | 6 | 6 |