Figure 3. A subset of Gdf7-lineage cells express neural stem cell markers.

(A–B″′) Many cells within the tumor tissue of Gdf7^Cre/+;SmoM2 mice coexpress neural stem cell marker Nestin (red) and glial marker GFAP (green). Arrows indicate co-localization. (C–E) Tumor cell lines can be invariably established from Gdf7^Cre/+;SmoM2 cerebella. (F–G″) These cells highly express multiple neural stem cell markers Nestin and Sox2 and can undergo serial passages. (H–J) Here, one representative colony is shown when cultured for 3 and 6 days. (K–N) Gdf7^Cre/+;SmoM2 cultured cells can differentiate into mature cerebellar cell types upon switching to culture media containing 10% FBS.