Figure 6. The competitive binding of polyphenols and anti-CD4 antibody to cell-surface CD4.

Fresh splenocytes were pre-incubated with various polyphenols. After blocking the Fc receptors, splenocytes were incubated with anti-CD3e-PE, anti-CD4-APC, anti-CD8a-FITC, or isotype-matched control mAbs. The interaction between cell-surface receptors and monoclonal antibodies was examined using FACS. Grey shading represents the control cells with control antibody; grey lines represent the control cells with functional antibody; black continuous lines represent the monomer phenylpropanoic acids-treated cells with functional antibody; and black dashed lines represent the polymerised polyphenols-treated cells with functional antibody. The data shown are representative of 3 independent experiments.