Figure 2. Megakaryocyte spreading on fibrinogen and α_IIbβ₃ expression.

(A) and (B) When plated on fibrinogen two populations of megakaryocytes are visible: half of the population spread regularly, while half showed abnormal spreading, with nuclei displayed towards cell periphery, a disordered distribution of actin and focal adhesion points more evident than stress fibres. (C) Flow cytometry showed decreased expression of α_IIbβ₃ on the surface of patient's megakaryocytes as compared with control megakaryocytes. *p<0.05 vs control.