Figure 4. Presence of tRK1 in msk1Δ mitochondria.

Mitochondria isolated from wild type or msk1Δ strains were treated with digitonin and MNase and total nucleic acids were separated on urea-acrylamide gel and analyzed by northern blot with respective probes. Total RNA represents the total cell RNA (2 µg) that was used to determine the levels of various tRNAs in the cytosol (Fig. 4A). Figure 4B represents the quantification of band intensities by densitometry.