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. 2012 Apr 23;7(4):e35258. doi: 10.1371/journal.pone.0035258

Figure 3. Identification of putative Ets-1 sites in the CCN2 promoter.

Figure 3

(A) The CCN2 proximal promoter (pictured are bases −293 to +1) contains eight putative Ets-1 binding sites (EBE 1–8) (open boxes), and these sites are in close proximity to the TRE (grey box) and SBE (black box). (B) Multiple mutation constructs were created containing individual substitutions of the EBE at positions 1–8 (ΔEBE 1–8) in the CCN2 proximal promoter that was cloned into a pGL3-firefly luciferase reporter construct.