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. 2012 Apr 5;2012:850836. doi: 10.1155/2012/850836

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Copyright © 2012 K. Tajiri and Y. Shimizu.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Accumulation of NKT cells in the NAFLD liver with high disease activity. (a) Immunohistochemical study using monoclonal antibody against for CD56 shows accumulation of CD56⁺ cells in the liver of NAFLD with the disease progresses. (FL; fatty liver versus NASH; nonalcoholic steatohepatitis). (b) The number of CD56⁺ or CD68⁺ cells. CD56⁺ cells are significantly increased as the disease progresses. (c) Flow cytometric analysis of isolated intrahepatic mononuclear cells with NAFLD. Numbers in the quadrant represent the percentage of positive cells. Right-upper quadrant represents NKT cells (CD3⁺CD56⁺ cells). (d) Flow cytometric analysis of Vα24 and intracytoplasmic cytokines of gated CD3⁺CD56⁺ cells among mononuclear cells isolated from livers with NAFLD. Numbers in each histogram represent the percentage of positive cells. These data have been previously presented in [30].