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. Author manuscript; available in PMC: 2012 Apr 24.
Published in final edited form as: Science. 2009 Sep 3;326(5950):285–289. doi: 10.1126/science.1178746

Fig. 1. Antigen binding properties of PG9 and PG16.

Fig. 1

(A) Binding of PG9 and PG16 to monomeric gp120 and artificially trimerized gp140 constructs as determined by ELISA. Antigens were coated directly onto ELISA wells in the experiments shown, but similar results were also obtained when antigens were captured onto wells with antibodies against non-competitive epitopes. (B) Binding of PG9 and PG16 to Env expressed on the surface of 293T cells as determined by flow cytometry. b12 is used as a control for ELISA assays. The bNAb b12, which binds with similar affinity to both cleaved and uncleaved forms of Env, and the non-neutralizing antibody b6, which only binds to uncleaved Env, are included in the cell surface binding assays to show the expected percentages of cleaved and uncleaved Env expressed on the cell surface (19). Experiments were performed in duplicate and data are representative of at least three independent experiments.