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. 2012 Feb 21;21(10):2357–2369. doi: 10.1093/hmg/dds056

Figure 3.

Figure 3.

Pax2.1 binds to the fadd promoter. (A) EMSA using fadd oligonucleotide probe containing the second pax2-binding site. Fadd probe bound to the pax2.1 protein (lane 2), which was displaced by unlabelled competitor (lane 3) and in the presence of pax2 antibody resulted in a supershifted (SS) complex. FP, free probe. Inset, western blot of in vitro translated full-length pax2.1 protein. (B) ChIP-PCR using Pax2 antibody (44 kDa protein detected by western blot of 36 hpf embryos). A fadd band (arrow) was specifically amplified from pax2 immunoprecipitates. IgG, negative control. Input corresponds to PCR from chromatin suspension without immunoprecipitation. (C) ChIP-PCR for Vax2 antibody (31 kDa protein detected by western blot of 36 hpf embryos). A fadd band (arrow) was specifically amplified from vax2 immunoprecipitates.