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. Author manuscript; available in PMC: 2012 Apr 24.
Published in final edited form as: Arthritis Rheum. 2011 Mar;63(3):713–721. doi: 10.1002/art.30156

Figure 3.

Figure 3

Competition immunoprecipitations confirm that human anti-HMGCR antibodies detect the C-terminus (panel A) and that the 200 kDa protein is not recognized by a unique autoantibody (panel B). A: Sera 10,009 and 9190 were preincubated with the indicated amounts of unlabeled C-terminal HMGCR and subsequently used to immunoprecipitate full-length 35S-methionine labeled HMGCR. B: Sera 9190 and 9176 were preincubated in the absence (lanes 11 & 13) or presence (lanes 12 & 14) of 300 ng unlabeled C-terminal HMGCR. They were subsequently added to radiolabeled lysates generated from HeLa cells treated with 10 µM mevinolin for 24 hours. The resulting immunoprecipitates were processed as described in the methods section. Identical data were obtained in 2 separate experiments using 4 (A) or 6 (B) different patient sera.