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. Author manuscript; available in PMC: 2012 Apr 24.
Published in final edited form as: Proteomics. 2010 Dec 15;11(2):202–211. doi: 10.1002/pmic.201000203

Figure 4.

Figure 4

Oxidation of Prx and subsequent activation of JNK/p38MAPK and inactivation of anti-apoptotic Bcl-2 family proteins following MDMA exposure. (A) Equal amounts (20 µg/well) of cytosolic proteins from vehicle-control or MDMA-exposed rat livers were separated on a 12% SDS-PAGE gel and subjected to immunoblot analysis using a specific antibody which recognizes either Prx (total) or oxidized Prx-SO3 (inactive form). (B,C) Equal amounts (20 µg/well) of cytosolic proteins from vehicle-control and MDMA-exposed rat livers were subjected to immunoblot analysis using the specific antibodies against JNK or phospho-JNK (B, left panel), p38K or phospho-p38K (B, right panel), Bcl-2 or phospho-Bcl-2 (C). (D) Cytosolic proteins (20 µg/lane; top) and mitochondrial proteins (20 µg/lane; bottom) were separated on a 12% SDS-PAGE gel and subjected to immunoblot analysis using a specific antibody against Bcl-XL.