Skip to main content
. Author manuscript; available in PMC: 2012 Jul 25.
Published in final edited form as: Angew Chem Int Ed Engl. 2011 Jun 10;50(31):7148–7152. doi: 10.1002/anie.201101552

Figure 4.

Figure 4

EPR spectra of cAD prepared under anaerobic conditions: a) apo-cAD reconstituted with Fe(II) to form di-ferrous enzyme; b) addition of heptanal to di-ferrous cAD; c) addition of heptanal and spin trapping reagent PBN to di-ferrous cAD. inset: expansion of g = 2 region of this spectrum showing PBN radical-adduct; d) addition of PBN only to di-ferrous cAD. e) spectrum of di-ferrous enzyme ion at 6 K (20 mW microwave power). All spectra except e) were recorded at 9.395 GHz; microwave power, 2 mW; modulation amplitude, 16 G; temperature, 77 K, and are plotted on the same scale.