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. 2011 Mar 3;2011:483851. doi: 10.4061/2011/483851

Figure 1.

Figure 1

Agaros gel electrophoresis of the p53 BstUI and the p53 MspI PCR products digested with BstUI and MspI restriction enzymes. (a) A1 allele does not create BstUI restriction site. The PCR product has 318 bp. Presence of CGCC sequence in A2 allele creates BstUI restriction site which leads to two fragments 182 bp and 136 bp. (b) A1 allele does not create MspI restriction site. The PCR product has 240 bp. Presence of CCGG sequence in A2 allele creates MspI restriction site which leads to two fragments 164 bp and 76 bp. Lanes 1, 6: 100 bp DNA ladder; lanes 2, 7: 1-1 homozygotes; lanes 3, 8: 1-2 heterozygotes; lanes 4, 9: 2-2 homozygotes; lane 5: no DNA in PCR reaction (blank).