Table 2. Primary antibodies.
| Target phenotypes | Target proteins/ Epitopes | Host | Dilution | Vendor |
|---|---|---|---|---|
|
Primary antibodies | ||||
| Neural stem cell and/or precursor |
Nestin (human, not rat/mouse-specific) |
Mouse |
1:400 |
Millipore |
| Neuronal precursor |
Doublecortin (DCX) |
Guinea pig |
1:3,000 |
Millipore |
| Neuron - early |
Type III b-Tubulin epitope J1 (Tuj1) |
Rabbit |
1:1,000 |
Covance |
| Neuronal precursor |
Doublecortin-like kinase (DCAMKL1) |
Rabbit |
1:50 |
Dr. Walsh, Harvard |
| Mitotic marker |
Ki67 antigen (NCL-Ki67p), Kiel University -raised antibody # 67 |
Rabbit |
1:1,000 |
Novocastra Labs |
| Human Nuclei -all |
Human nuclear protein epitope (HNu) |
Mouse |
1:1,200 |
Millipore |
| Neuron -human |
Synaptophysin, human, not rat/ mouse –specific(Syn) |
Mouse |
1:800 |
Millipore |
| Muller glia -activated |
GFAP |
Rabbit |
1:700 |
DAKO |
| Photoreceptor |
Recoverin (Rec) |
Rabbit |
1:1,000 |
Millipore |
| Rod photoreceptor |
Rhodopsin, Rho4D2 |
Mouse |
1:100 |
Dr.Molday (UBC) |
| Microglia |
Ionized calcium binding adaptor molecule-1 (Iba-1) |
Rabbit |
1:200 |
Waco Chemicals USA |
|
Secondary antibodies | ||||
| Alexa Flour 488 |
Goat anti-Rabbit |
Goat |
1:400 |
Invitrogen |
| Alexa Flour 594 | Goat anti-Mouse | Rabbit | 1:400 | Invitrogen |