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. 2012 Apr 24;7(4):e35782. doi: 10.1371/journal.pone.0035782

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Boumart et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

In vitro invasion and intracellular multiplication were performed in epithelial cell lines (HT29 and LMH) and macrophages (HD11 and J774). Persistent (P) (SS308, SS209) and non-Persistent (NP) (SS304, SS278) strains were analyzed in addition to a human isolate (H) (SS291). Cells were infected at a MOI of 10 CFU/cell and the number of intracellular bacteria was determined 2 h (black) and 20 h (grey) post-infection. Data representing the mean ± SD of at least two independent experiments carried out in duplicate. The invasive strain of Salmonella Enteriditis LA5 was used as a positive control.