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. 2012 Apr 24;7(4):e36121. doi: 10.1371/journal.pone.0036121

Figure 5. Lin28 expression is controlled by miR-125b in differentiating hESCs.

Figure 5

Untransfected hESCs (Cntl) or hESCs transfected with anti-miR-125b inhibitor (anti-125b) were cultured in differentiation medium for 2 or 8 days. A) Cells were analyzed for expression of miR-125b by qPCR. Appropriate downregulation of mature miR-125b was seen in both undifferentiated and differentiating hESCs. Data shown are mean±s.e.m. (N = 3). **, p<0.01; ***, p<0.001. B) Cell lysates were assayed for Lin28 by immunoblot analysis compared to undifferentiated cells (Undiff). Lin28 protein expression was noticeably decreased in hESCs differentiated for 2 or 8 days compared to undifferentiated cells, while transfection with anti-125b induced Lin28 expression in undifferentiated as well as differentiating cells (TOP). Actin was used as a loading control. Representative results are shown. Quantitation of fluorescent signals is shown (BOTTOM). Data shown are mean±s.e.m. (N = 3). *, p<0.05; **, p<0.01; ***, p<0.001.