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. 2012 Apr 24;7(4):e35866. doi: 10.1371/journal.pone.0035866

Figure 5. Exogenous expression of Tr/E significantly decreases mRNA expression of ISG56 in Ad/Tr-cells following polyI∶C stimulation.

Figure 5

HEC-1A cells were either treated with medium alone or with MOI 50 of Ad/dl or Ad/Tr and incubated in presence of media or 25 µg/ml of polyI∶C. (A) At 6 h post treatment, total RNA was harvested and mRNA levels of ISG56 were assessed by conventional RT-PCR. (B) Quantification of ISG56 expression using ImageQuant software. Values are normalized to a housekeeping gene 18S in the same sample and presented as relative fold induction over untreated cells, shown in arbitrary units. The data are representative of at least two independent experiments performed in triplicate and are shown as the mean ± SD. Statistical analysis was performed using Student's t test with * representing significant difference between the groups, p<0.05.