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. 2012 Apr 24;7(4):e35663. doi: 10.1371/journal.pone.0035663

Figure 1. Live-cell imaging of GFP-Rab20 in RAW264 macrophages during phagocytosis of IgG-Es.

Figure 1

Live RAW264 cells expressing GFP-Rab20 were put into contact with IgG-Es and observed by phase-contrast and fluorescence microscopy. Time-lapse images were acquired using the MetaMorph imaging system. Phase-contrast images are shown (upper panels). The elapsed time is indicated at the top. The binding of IgG-Es to the cell surface was set as time 0. It is noteworthy that Rab20 was associated with newly formed phagosomes (arrows). At least four cells were examined in four independent experiments. Similar results were obtained from four independent experiments. Scale bars: 5 µm. The corresponding video is Movie S1.