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. 2012 Apr 24;7(4):e35824. doi: 10.1371/journal.pone.0035824

Figure 2. Characterization of hiPSC generated either from fibroblasts or CB-CD34+ HSPCs.

Figure 2

All the hiPSC lines generated from either fibroblasts or CB-CD34+ HSPCs express the pluripotency markers Oct4, Sox2, Nanog and Rex1 (A), the pluripotent surface markers Tra-1-60, Tra-1-81, SSEA-3 and SSEA-4, as well as nuclear Oct4 (B) and display an euploid karyotype (C). All hiPSC lines differentiated in vivo (teratoma formation) (D) into tissues representing the three germ layers: ecto-, meso- and endoderm. Immunocytochemistry pictures are shown at 20× magnification. (E) RT-PCR confirming that CB-iPSC#2 and F-iPSC#2 did not silence OKSM factors after >20 passages.