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. 2012 Apr 24;7(4):e35933. doi: 10.1371/journal.pone.0035933

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Eamens et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Photo-bleached phenotypes expressed by Col-0 and drb mutants transformed with the amiR^164B-PDS plant expression vector. Scale bars = 7.5 mm. (B) amiR-PDS accumulation and PRI-MIR164B-PDS and PDS expression in amiR^164B-PDS expressing plants. (C) amiR^169A-PDS-directed PDS silencing in wild-type plants and drb mutants. Red arrows indicate the small sectors of photo-bleaching displayed by drb235/amiR^169A-PDS plants. Scale bars = 7.5 mm. (D) amiR-PDS accumulation and target transcript expression in plants expressing the modified PRI-MIR169A transcript targeting PDS for amiRNA-directed silencing.