Figure 5. Host stromal cell-derived factors synergistically enhance RA-DC TLR5 expression.

Control DCs and RA-DCs were treated with intestinal stromal cell-conditioned medium (SCM 250 µg/ml), TGF-β (10 ng/ml), TGF-β inhibitor (SB-505124 1 µM) or SCM plus TGF-β inhibitor for 24 h, and TLR5 expression was determined by flow cytometry (a). Shaded histogram, control DCs; solid line, RA-DCs; dotted line, treated control DCs (left) or RA-DCs (right). Data are representative of three experiments. (b) Aggregate data of DC TLR5 MFI with mean ± SEM of three experiments. *P<0.05; compared to untreated DCs. One wild type mouse for each experiment.