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. 2012 Apr 24;7(4):e35587. doi: 10.1371/journal.pone.0035587

Figure 3. Expression of mRNA and antiviral MxA protein in response to GRW/Aa infection.

Figure 3

A549 cells were infected at multiplicity of infection 1. Samples were taken at indicated time points post infection. A) Induction of IFN-β, IFN-λ1 and MxA mRNA (shown as fold of increase in comparison with uninfected cells) in response to GRW/Aa infection measured as virus genome copies by qPCR. Cells stimulated with poly I:C were transfected with 1.6 µg of poly I:C six hours prior infection. Experiment was performed three times. Data are presented as the mean ± SD of the mean. B) Expression of antiviral MxA protein detected by Western blot. NC (negative control), uninfected A549 cells. PC (positive control), A549 cells infected with HTNV for 4 days. Representative results of two independent experiments are shown.