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. 2012 Apr 24;7(4):e35094. doi: 10.1371/journal.pone.0035094

Figure 13. Ability to induce intra-tumoral lymphangiogenesis was abrogated by both VEGF-D and α9 integrin knock down in 468LN cells:

Figure 13

(A, B) Intra-tumoral lymphangiogenesis was measured indirectly from the expression of murine lymphatic endothelial marker LYVE-1 in tumors at the mRNA level with qRT-PCR (A) and protein level with western blot (B). Murine LYVE-1 expression was very high in 468LN tumors and this expression was nearly completely abolished in both Δα9/468LN and ΔVEGF-D/468LN tumor implants. Data were derived from 16 tumors. Four tumors in a single mouse were pooled to extract RNA and proteins, showing the data in four mice; Matrigel was retrieved from four implants. Data represents mean (n = 4) ± S.E, *p<0.05, *p<0.001.