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A, The TI-2 response was evaluated by measuring TNP-specific IgM and IgG3 serum levels after i.p. immunization with 25 µg TNP-Ficoll. Mice were bled at the indicated time points and serum antibody isotypes were measured by ELISA, (n = 5). B, Elispot analysis of TNP-specific-IgM-producing cells in response to TNP-Ficoll seven days post i.p. immunization (n = 4). *p≤0.05, **p≤0.01, ***p≤0.001.
