Skip to main content
. 2012 Apr 24;7(4):e35511. doi: 10.1371/journal.pone.0035511

Figure 5. The effect of TNFSF13 exposure on intracellular pH, and of decreased intracellular pH on TNFSF13 and FAS receptor gene expression.

Figure 5

Intracellular pH of U-87 MG cells was artificially decreased using nigericin and phosphate buffer to pH 6.4, 6.9 and 7.3 for 0.5, 3, 12 or 24 hours and transcript levels of the TNFSF13 (A) or FAS receptor (B) genes measured by qRT-PCR. Expression levels were calculated relative to three housekeeping genes (β-actin, TATA box binding protein and ubiquitin C) using the deltadelta Ct method. aTranscript levels at the 0.5 hours time point were significantly greater than at all other time points, bDecreasing pH significantly suppressed transcript levels of TNFSF13, cThere was no significant effect of pH on FAS receptor transcripts. U-87 MG glioblastoma cell cultures were treated with TNFSF13 (1 pg or 100 pg TNFSF13/microliter of culture media) for up to 48 hours and the intracellular pH determined (C). *p<0.05, ***p<0.001.