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Measurements were made in the presence (black columns) and absence (grey columns) of exogenous CPR. (A) HO activity measured in cytosolic fractions from Sf9 cells expressing the indicated HO variants. (B) Same experiments made in homogenates from Sf9 cells. (C) Enzyme activity assay after purification using the Strep-tag/Streptavidin system. Data are shown as means ± SEM (n = 3, *p<0.05).
