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Measurements were made in the presence (black columns) and absence (grey columns) of exogenous CPR. (A) Activity measured in the cytosolic fractions from Sf9 cells expressing the HO mutants. (B) Measurement in homogenates from infected Sf9 cells. (C) Enzyme activity assay after purification using the Strep-tag/Streptavidin system. Data are shown as means ± SEM (n = 3, *p<0.05).
