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. 2012 Apr 24;7(4):e35483. doi: 10.1371/journal.pone.0035483

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Linnenbaum et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) S-HO-1ΔC266 (B) S-HO-2ΔC289 (C) S-HO-1 (D) S-HO-2 (E) S-HO-1-CPR co-purification (F) S-HO-2-CPR co-purification. The elution volume of peak 1 is nearly the void volume of the column and corresponds to sizes >2000 kDa. Peak 2 corresponds to a size in the range of 40 kDa. Western Blots of the purified samples (P) and the gel filtration-peaks are shown in the small boxes, detected with the HO-1 antibody (A+C+E) or HO-2 antibody (B+D+F), respectively.