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. 2012 Apr 24;7(4):e35483. doi: 10.1371/journal.pone.0035483

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Linnenbaum et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HEK293 cells were transfected with YFP-HO-1 (A-row 1) and YFP-HO2 (B-row 1) or co-transfected with YFP-HO1 and CPR-CFP (A-row 2) or YFP-HO2 and CPR-CFP (B-row 2). Cells were analyzed under normoxic conditions using the CFP-channel (column 1) and YFP-channel (column 2) or after hypoxia in the CFP-channel (column 3) or YFP-channel (column 4). The red arrows in panel A point to typical cells where the translocation of HO-1 (row 1) and its prevention in the presence of CPR (row 2) are visible. The white bars correspond to 10 µm.