Figure 1. Silencing efficiency was detected after ITGB4 siRNA-containing lentivirus was transfected.

The effective ITGB4 siRNA lentivirus vector and control nonsense siRNA lentivirus vector were prepared and transfected into 16HBE14o- cells. (A) 72 h after lentivirus vector transfection, ITGB4 mRNA expression was detected by real time PCR. (B) Western blot analysis was used to detect ITGB4 protein expression 72 h after tansfection. (C) Quantification of western blot normalized to the level of GAPDH in Figure 1A.