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. 2012 Apr 24;7(4):e32060. doi: 10.1371/journal.pone.0032060

Figure 4. T cell proliferation was observed after coculturing T cells with control transfected 16HBE14o- cells or ITGB4-silenced cells.

Figure 4

(A) On the basis of decreased CSFE staining, T cell proliferation are shown as percentage of proliferating cells, and are representative of one experiment repeated six times. Percentage of dividing cells (cells that have undergone 1 division) is shown for control silenced 16HBE14o- cells +T cells and ITGB4 silenced 16HBE14o- cells +T cells cocultures. Proportion of original population induced to divide after stimulation was determined by measuring the number of events in each cell division. (B) Meanwhile, T cell proliferation was assessed by a BrdU incorporation assay after coculture. The data are expressed as the mean (±SEM) values of the specific absorbance from 10 independent experiments.