Fig. 1.

MG development of female offspring in a late-life effects cross-foster study. (A) Whole mount preparations of mammary tissue from female offspring are shown at PND 22 (25×; a lymph node appears as a large darkly staining object), PND 42 (50×), and PND 63 (50×). Glands pictured are representative of mean respective scores (N = 10–13 adult females per treatment group at PND 22, N = 9–18 per group at PND 42, N = 9–17 per group at PND 63). *Significant treatment effect by ANOVA, compared to control; p < 0.05. (B) On the left, whole mounts from representative adult female offspring at 18 months of age (16×). Large arrows indicate unusual, darkly staining foci; one small arrow in 5U indicates peripheral, localized increases in epithelial density observed in some PFOA-exposed animals at 18 months. On the right, histopathologic images from contralateral glands in the same animal show ductal areas that account for darkly staining foci observed on whole mounts (400×; N = 5–12 females per group). This figure was reproduced with permission from Elsevier, Inc., and is excerpted from [64].