Figure 1.

Enforced USP2a^WT expression enhanced tumorigenesis in T24 human bladder cancer cells. (A) Proliferation rate in T24 bladder cancer cells transiently engineered to express USP2a^WT, T24-USP2a^WT and Vec (vector only), T24-Vec. Cells were serum starved for 16 h before changing into growth medium. At the indicated time points, cells were fixed and stained with crystal violet solution. (B) Cell migration capability of T24-USP2a^WT and T24 Vec cells were analyzed using gelatin-coated invasion chamber kit (company) as described in the Materials and Methods section. (C) Migration was compared in T24-USP2a^WT and T24 Vec cells by a wound-healing assay. (D) T24-USP2a^WT and T24-Vec cells were seeded at low density and incubated for colony formation for 7 d. After fixing and staining cells, number of colonies was counted. Representative images were shown. Asterisks in this figure indicate p < 0.05.