Figure 2.

USP2a^WT altered activation of signal transduction. (A) Erk/MAPK phosphorylation in USP2a^WT expressing T24 cells treated with recombinant HB-EGF (100 ng/ml). Protein from whole-cell lysates at the indicated times were blotted with the Abs against p-Erk/MAPK, Erk/MAPK and β-actin. (B) TUNEL assay showed less apoptotic cells in USP2a-expressing cells compared with control. (C) Levels of cleaved PARP (c-PARP) were detected in whole lysates from T24-USP2a^WT and T24-Vec cells incubated in serum free medium (SF), TPA, nocodazole, cisplatin or CHX for 24 h.