Figure 2.

P-ΔNp63α regulated the luciferase activity driven by the autophagic gene promoters upon cisplatin treatment. ΔNp63α-wt cells (A) and ΔNp63α-S385G cells (B) were transfected for 24 h with 100 ng of the LightSwitch_Pro reporter plasmids containing the indicated autophagic promoters or with 100 ng of the control promoter-less reporter plasmid. Cells were exposed to control medium without cisplatin (Con) and medium with 10 µg/ml cisplatin (CIS) for 12 h. RenSP Renilla luciferase reporter activity assays were conducted in triplicate (±SD are indicated, p < 0.05). Data presented as relative to data obtained from the control untreated cells containing the promoter-less reporter plasmid designated as 1.