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Cisplatin treatment modulates protein levels of autophagic markers through miRNA-dependent regulation. ΔNp63α-wt cells were transfected with the mock (scrambled) miRNA and specific miRNA mimics or inhibitors for 24 h. Cells were treated with control medium without cisplatin (Con) or medium with 10 µg/ml cisplatin (CIS) for additional 16 h and then tested for protein levels of the indicated endogenous autophagic markers. Loading levels were tested using an antibody against β-actin.
