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. 2011 Dec 15;12(12):1050–1058. doi: 10.4161/cbt.12.12.18142

Figure 4.

Figure 4

IDO deficiency accentuates the effects of inflammatory insults that promote colon carcinogenenesis. (A) Colitis induction. Colons from WT and IDO-/- mice treated 3 d with 3% DSS in drinking water were fixed in paraffin and processed for H&E staining. Representative examples scored for no colitis, mild colitis and severe colitis are shown at original magnifications of 10× (middle) or 20× (bottom). (B) Colon carcinogenesis. WT and IDO-/- mice were treated once i.p. with 20 mg/kg DMH and then 7 d 2% DSS in drinking water followed by normal drinking water to an endpoint of 18 wks. Colons were harvested from euthanized mice, fixed in paraffin and processed for H&E staining. Representative images of gross pathology in WT or IDO-/- mice (top panels) illustrate observations of macroscopic tumors (arrows) or prolapsed rectums (arrowheads). Representative images of histopathology in IDO-/- mice (bottom panels) illustrate observations of (a) no tumor, (b) mucosal invasion, (c) submucosal invasion and (d) muscular invasion. Original magnifications are 4× (top), 10× (middle), 20× (bottom).